By default, when you double-click on a node in your workspace, a graph window will open displaying the forward scatter-area (FSC-A) and the side scatter-area (SSC-A) on the X and Y-axis of the plot, respectively. Your cells will be displayed as events along an X and Y-axis (as part of a bivariate plot), allowing you to identify and gate different populations. To open a new Graph Window, double-click on any population node within the sample list in the workspace. These menus allow you to change the type of graphical display (density plots, contour plots, histograms, etc.), the color, shading, and behavior of gates, and permit addition of a wide range of statistics. For more information about axis transformation and scaling, click here.Īt the bottom of the graph window are three expandable menus Options, Active Gate, and Statistics. A transform button sits adjacent to each axis drop-down menu to modify the axis scaling. The primary graphical display has two drop-down menus (one for each axis) for viewing your cells under any acquired parameter (FITC, APC, PE, Cy5, FL-2, etc). For more information on the Breadcrumb Bar see below. The Breadcrumb Bar provides a reference to where you are in your gating hierarchy, and has quick links to ancestral gates. For more information about the types of gates available, click here. The Options, Active Gate, and Statistics expandable menus.Īt the top of the window are the available tools for creating gates.The Graph Window consists of several important components: The Graph Window provides an interface to begin gating your populations, adding statistics, and is the first step in creating your heirachical tree in the workspace. Quickly gate, navigate, and add statistics to your populations using FlowJo’s intuitive menu bars and icons.ĭata analysis in FlowJo begins with the Graph Window. Begin data analysis in FlowJo with the user- friendly Graph Window.
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